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Method for determination of angiotensin-converting enzyme activity in blood and tissue by HPLC
Summary
Type:
HPLC
Application #:
107810
Detection:
UV, 228nm
Conditions:
eluent: methanol - 10mM KH2PO4 (1:1), adjusted to pH 3.0 with phosphoric acid
flow rate 1.0ml/min
Authors:
Horiuchi M
Source:
J Chromatogr 233 (1982) 123 - 130
Details
Legend: Fig. 1. Chromatograms obtained from various samples incubated with (upper traces) or without (lower traces) Hip-His-Leu; peaks: 1. His-Leu, 2. hippuric acid, 3. Hip-His-Leu.
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